Comparative evaluation antimicrobial and antitumor activities of natural colostrum peptide and its synthesized analogue

Abstract

Antimicrobial and antitumor activities of bovine colostrum peptide and its synthesized analogue were carried out. The peptide was produced by standard solid phase synthesis followed by purification by high-performance liquid chromatography. Peptide purity and primary structure were confirmed by MALDI and ESI MS. It was found that the synthesized peptide with ACSAG amino acid sequence and a molecular weight of 430.2332 was an analogue of the natural one. The synthesized peptide had a high hydrophilic value and isoelectric point of 5.22. The obtained data theoretically confirm that the studied peptide belongs to a class of antimicrobial peptides. It was experimentally established that both natural and synthesized peptides had antimicrobial activity against E. coli ATCC 25922, P. aeruginosa 27/99 and B. subtilis АТСС 6633, antifungal and anti-tumor activity against C6 cells. After 48 h there was a significant 50% decrease in the tumor cells population at the concentration of the natural peptide of 365.5 ± 3.8 mg.ml-1, and the synthesized peptide concentration of 312.7±3.5 mg.ml-1 in the nutrient medium. This synthesized peptide has a greater biological activity and can be effective at lower concentrations, than the natural one. © 2024 University of Food Technologies Plovdiv. All rights reserved.